Points - Recent Research
Wild Ginseng Attenuates Anxiety and Depression-Like Behaviors during Morphine Withdrawal
Xia Ku Cao Chemopreventive Effect of a Mixture of Chinese Herbs (Antitumor B) on Chemically Induced Oral Carcinogenesis
Transcriptional Profiling of Human Skin Fibroblast Cell Line Hs27 Induced by Herbal Formula Astragali Radix and Rehmanniae Radix

Wild Ginseng Attenuates Anxiety and Depression-Like Behaviors during Morphine Withdrawal

Lee B, et al. Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Korea.

The purpose of this study was to evaluate whether wild ginseng (WG) administration could attenuate Anxiety- and depression-like behaviors and expression of corticotrophinreleasing factor (CRF) and neuropeptide Y (NPY) following withdrawal from repeated morphine administration in rats. Male rats were administered daily doses of WG (50, 100, or 200 mg/kg, i.p.) for 5 days, 30 min before morphine injection (40 mg/kg, s.c). The Anxiety- and depression-like behavioral responses were measured 72 h after the last morphine injection using an elevated plus maze (EPM) and forced swimming test (FST), respectively. Changes in hypothalamic CRF and NPY expressions were also examined by analyzing their immunoreactivities in the hypothalamus. Daily administration of WG significantly reduced Anxietyand depression-like behavior, and elicited the suppression of CRF expression and the stimulation of NPY expression in the hypothalamus. Our results demonstrated that WG extract might be effective at inhibiting the Anxiety and depression responses due to morphine withdrawal by possibly modulating the hypothalamus CRF and NPY systems. Furthermore, these findings imply that WG extract can be used for developing new medication to cure or alleviate morphine withdrawal symptoms and to prevent relapses of morphine use.

J Microbiol Biotechnol.2011 Oct;21(10):1088-96.

Source: PubMed

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Chemopreventive Effect of a Mixture of Chinese Herbs (Antitumor B) on Chemically Induced Oral Carcinogenesis

Wang Y, et al. Department of Surgery, Washington University School of Medicine, St. Louis, Missouri.

In this study, we evaluated chemopreventive efficacy of Antitumor B, a Chinese herbal mixture of six plants (Sophora tonkinensis, Polygonum bistorta, Prunella vulgaris, Sonchus arvensis L., Dictamnus dasycarpus, and Dioscorea bulbifera) on the development of 4-nitroquinoline-1-oxide (4NQO) induced oral squamous cell carcinomas in A/J mice. Antitumor B, delivered through diet, inhibited 4NQO-induced oral cancer development by 59.19%. The reduction of cell proliferation appears to be associated with efficacy of Antitumor B against 4NQO-induced oral cancer in A/J mice. The expression of epidermal growth factor receptor (EGFR) and phosphorylated EGFR (Tyr1173) were down-regulated by Antitumor B. Tissue distribution of Antitumor B was determined using obacunone, matrine, and maackiain as marker chemicals. We found significant amounts of obacunone, matrine, and maackiain in the blood after 1-wk treatment. The concentrations of these three compounds did not increase further at 18 wk, suggesting that plasma concentrations had reached a steady-state level at 1 wk. There was no significant body weight loss and there was no other obvious sign of toxicity in Antitumor B-treated mice. These results suggest that Antitumor B is a promising agent for human oral cancer chemoprevention.

Mol Carcinog. 2011 Nov 15. doi: 10.1002/mc.20877.

Source: PubMed

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Transcriptional Profiling of Human Skin Fibroblast Cell Line Hs27 Induced by Herbal Formula Astragali Radix and Rehmanniae Radix

Zhang Q, et al. Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong.

ETHNOPHARMACOLOGICAL RELEVANCE: The herbs Astragali Radix (AR) and Rehmanniae Radix (RR) have long been used in traditional Chinese Medicine and serve as the principal herbs in treating diabetic foot ulcer. AIM OF THE STUDY: Chinese herbal formulus comprising Astragali Radix (AR) and Rehmanniae Radix (RR) have been shown to improve the healing of diabetic foot ulcer through enhancing the viability of primary fibroblasts in diabetic patients suffering insulin resistance. Our previous study demonstrated that the herbal formula NF3 comprising of AR and RR in the ratio of 2:1 was effective in promoting wound healing in diabetic rats, and in vitro data indicated that the wound healing effects of NF3 might be due to the regulation and coordination of inflammation, angiogenesis and tissue regeneration. However, the underlying molecular mechanism has not been well investigated. In this study, we investigated the cellular and molecular effects of the herbal formula NF3 on human skin fibroblast cells. MATERIALS AND METHODS: Human skin fibroblast cells Hs27 were treated with NF3 ranging from 0 to 8mg/ml for 24h, and the cells without NF3 treatment were used as control. Cell proliferation assay and cell cycle analysis were performed. Transcriptional profiles of Hs27 cells upon NF3 treatment were acquired by using a human cDNA microarray containing 10,000 genes, and the signaling pathways differentially regulated by NF3 were identified and analyzed. RESULTS: NF3 promoted Hs27 cell proliferation and cell cycle progression. Microarray analysis revealed that 116 genes were differentially expressed upon NF3 treatment. Functional analysis of the genes indicated that NF3 mainly activated Wnt and angiogenesis related pathways, which are directly related to cell proliferation, angiogenesis, extracellular matrix (ECM) formation and inflammation during the process of wound healing. CONCLUSION: This study provides insight into the molecular mechanism of how the herbal formula Astragali Radix and Rehmanniae Radix may serve as potential therapeutics for wound healing.

J Ethnopharmacol. 2011 Oct 30.

Source: PubMed

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