Acupuncture Improves Cognitive Deficits and Increases Neuron Density of the Hippocampus in Middle-Aged SAMP8 Mice
Li G, et al. Department of Rehabilitation Medicine, First People's Hospital of Chenzhou, Chenzhou, Hunan Province, China.
OBJECTIVES: To examine whether acupuncture could improve cognitive deficits and reduce the loss of neurons in mice models of ageing. METHODS: Male 7.5-month-old senescence-accelerated mouse prone 8 (SAMP8) and age-matched senescence-resistant inbred strains 1 (SAMR1) were divided into four groups (n=15 per group): SAMP8 acupuncture group (Pa), SAMP8 non-acupuncture point control group (Pn), SAMP8 control group (Pc) and SAMR1 normal control group (Rc). The behaviours were examined by the Morris water maze test and the neuron density in the hippocampus was estimated by the optical fractionator technique. RESULTS: The Morris water maze test demonstrated that the cognitive deficits of SAMP8 mice were improved by acupuncture treatment. Neuronal loss was found in hippocampal regions CA1 (-24%), CA3 (-18%) and DG (-28%) of Pc compared with Rc. The neuron number in hippocampal CA3 and DG of the Pa
group was significantly increased by therapeutic acupuncture compared with the Pc group. CONCLUSIONS:
Acupuncture improved the cognitive impairment of middle-aged SAMP8 mice which could be attributed to the
reduced neuron loss in hippocampal regions CA3 and DG. These results suggest that reducing neuron loss in
the hippocampus by acupuncture is a potential therapeutic approach for the treatment of Alzheimer's
disease and cognitive impairment diseases.
Acupunct Med. 2012 Sep 12.
The Effects of Acupuncture on the Inner Ear Originated Tinnitus
Rogha M, et al. Assistant Professor, Department of Otolaryngology-Head and Neck
Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
BACKGROUND: Tinnitus is a chronic and bothering problem which in some patients may lead to some psychological reactions. While tinnitus may be caused due to some definable structural abnormalities, sometimes no structural etiologic defect can be found. This study aimed to evaluate the therapeutic effects of acupuncture on the inner ear originated tinnitus in the latter mentioned group. METHODS: This prospective clinical trial study has been done in Alzahra and Kashani hospitals in Isfahan, Iran during 2010-2011. Simple sampling was used to select patients who were then divided into two groups of true acupuncture group and placebo group. The number of patients in each group was 27. The hospital anxiety and depression scale (HADS), tinnitus severity index questionnaire, and tinnitus loudness scale were completed by the patients. Two latter questionnaires were completed again after the 5th and 10th sessions of acupuncture treatment. The case group was treated with effective acupuncture with true acupuncture needles while the control group was treated ineffectively, i.e. similar to the usual acupuncture method but with fake needles. Independent t-test was used to compare the mean of tinnitus severity index and loudness scores between the two groups. We also used repeated measures ANOVA test to compare the mean of tinnitus severity index and loudness for different assessments in either group. RESULTS: After the 5th and 10th sessions of treatment, the mean of tinnitus severity index reduced significantly only in the case group (p = 0.002, and p = 0.001, respectively). In addition, the quality of life in the case group also improved after the treatment. Moreover, the mean of tinnitus loudness also reduced significantly only in the case group after 5 and10
sessions of treatment (p = 0.001, and p < 0.001, respectively). CONCLUSIONS: It seems that acupuncture
can improve tinnitus in some selected patients.
J Res Med Sci.2011 Sep;16(9):1217-23.
The Roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen)
Inhibit Atherogenic Events
Wing-Shing Cheung D, et al. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Phytochemistry
and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
ETHNOPHARMACOLOGICAL RELEVANCE: The roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) are principle herbs of Chinese herbal formulae which have long been used to treat cardiovascular diseases. AIM OF STUDY: The present study validated the anti-atherogenic effects of three extracts, Danshen alone (DE), Gegen alone (GE) as well as DGE and interpreted their combination effects statistically. MATERIALS AND METHODS: The anti-atherogenic effects of the three extracts were studied in three assays with regards to inflammation, foam cell formation and vascular smooth muscle cell (vSMC) proliferation using lipopolysaccharide (LPS)-induced nitric oxide production model, macrophage foam cell formation model and platelet-derived growth factor (PDGF)-induced vSMC proliferation model, respectively. The combination effects of DGE were statistically analyzed using combination index (CI) and fixed-ratio experimental design. RESULTS: The anti-atherogenic effects of the three extracts including anti-inflammation, anti-foam cell formation and anti-vSMC proliferation were demonstrated in this study. Their combination effects in anti-inflammation, anti-foam cell formation and anti-vSMC proliferation were found to be synergistic, additive and antagonistic, respectively. CONCLUSIONS: This study provided scientific support for the combination use of DGE on atherosclerosis and presented one
of the first applications of statistical interpretations of the combination
effects of the 2-herb formula.
J Ethnopharmacol.2012 Aug 19.